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A Simple Method to Confirm and Size Deletion, Duplication, and Insertion Mutations Detected by Sequence Analysis

โœ Scribed by Lawrence N. Hjelm; Ephrem L.H. Chin; Madhuri R. Hegde; Bradford W. Coffee; Lora J.H. Bean


Book ID
118235038
Publisher
American Society for Investigative Pathology
Year
2010
Tongue
English
Weight
227 KB
Volume
12
Category
Article
ISSN
1525-1578

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The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqu