A simple method of lower extremity arteriography in the rat

✍ Scribed by N. Berber Oldenbeuving; Ashish K. Jain; Byron D. Poindexter; Beatrice Engels; Martha J. Wilkins; Luis O. Vasconez

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 130 KB
• Volume: 18
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/(sici)1098-2752(1998)18:1<33::aid-micr8>3.0.co;2-v

✦ Synopsis

Microarteriography is commonly used to evaluate the patency of small vessel anastomoses. Several relatively simple methods of microarteriography have been described, but none provide enough detail of the operative technique to allow the novice to perform this procedure. The authors provide a detailed simplification of the technique as described by Zhang et al. 1 utilizing materials that are inexpensive, reusable, and commonly found in the laboratory setting. After approval by the Institutional Animal Care and Use Committee, six retired-breeder laboratory rats were anesthetized and underwent endoscopic microanastomosis of the femoral artery. Seven-day patency was evaluated by arteriography as described herein. High-quality X-ray images and slides were obtained with this technique by a novice laboratory surgeon.