A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu
โฆ LIBER โฆ
A simple method for the preparation of large quantities of pure plasmid DNA
โ Scribed by G.O. Humphreys; Geraldine A. Willshaw; E.S. Anderson
- Book ID
- 115746140
- Publisher
- Elsevier Science
- Year
- 1975
- Weight
- 423 KB
- Volume
- 383
- Category
- Article
- ISSN
- 0005-2787
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