A simple method for the determination of isoelectric points of ampholytes with closely spaced pKa values using pressure-mediated capillary electrophoresis

A rapid method for pKa determination of

A rapid method for pKa determination of drugs using pressure-assisted capillary electrophoresis with photodiode array detection in drug discovery

✍ Yasushi Ishihama; Masahiro Nakamura; Toshinobu Miwa; Takashi Kajima; Naoki Asaka 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB 👁 1 views

We developed a rapid screening method for determination of pK(a) of candidate drugs by pressure-assisted capillary electrophoresis (CE) coupled with a photodiode array detector. Application of pressure during CE analysis allowed completion of one CE run in less than 1 min, and the obtained pH-metric