We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m
โฆ LIBER โฆ
A simple method for the assay of bile salt sulfotransferase using glycolithocholate coupled to sepharose
โ Scribed by Lee-Jing Chen
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 304 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A simple procedure is described for measuring bile sait sulfotransferase. The system used glycolithocholate coupled to Sepharose as substrate. The assay is carried out directly in the scintillation counting vials. It is easy to perform and reproducible; and the results are comparable to those obtained from other established procedures. There is no thin-layer chromatographic separation of the reaction products.
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