𝔖 Bobbio Scriptorium
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A simple method for subcloning DNA fragments from gel slices

Book ID
113289075
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
145 KB
Volume
6
Category
Article
ISSN
0168-9525

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πŸ“œ SIMILAR VOLUMES

A simple method for filter hybridization
A simple method for filter hybridization of labeled restriction fragments excised from gels
✍ Renzo Marotta; Marion Bremenkamp; Francesco Salamini πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 403 KB

Labeled DNA restriction fragments excised from agarose or bisacrylylcystamine-acrylamide gels can be used for hybridization to nitrocellulose-bound DNA without eliminating the gel matrix. A gel slice containing the labeled fragment is excised, dissolved by heating at 105 degrees C (in the presence o