โ Scribed by E. Lorch; G. Rentsch
No coin nor oath required. For personal study only.
I n similar tests with isolated fat cells from various sources, the total content of triglyceride, in the same way as the amount of DNA which is contained, does not lend itself for use as a basis for comparison. If the number of cells used in the experiment is adopted as a basis for comparison, it is very difficult to distinguish between metabolically active and metabolically inactive cells, just as it is difficult to distinguish between cells and fat droplets. --When stained with acridine orange and a simple cell counting technique used, the fat cells can be counted quickly, and if desired, the count can be repeated with a reasonable degree of accuracy.
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This paper presents a theoretical justification for usine 3-hydroxy[3-"Clbutyrate to label the intramitochondrial pool of acetyl CoA and hence for calculating the percentage of citrate which is oxidized by the tricarboxylic acid cycle. Knowledge of this percentage can be used to calculate the rate o