✦ LIBER ✦
A simple method for rapid purification of avian sarcoma virus supercoiled DNA by selective precipitation of infected cell chromatin
✍ Scribed by Ramareddy V. Guntaka
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 404 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple and effective method to partially purify virus-specific DNA from avian sarcoma virus-infected QT6 cells has been developed. This method consists of lysing infected cell nuclei in water followed by precipitation of the resulting chromatin with sodium chloride. More than 98% of the host cell DNA could be removed by this method without diminishing viral DNA yields. This method is equally applicable to SV40 DNA purification.