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A simple method for monitoring the cysteine content in synthetic peptides

✍ Scribed by Kata Horváti; Szilvia Bõsze; Ferenc Hudecz; Hedvig Medzihradszky-Schweiger

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
156 KB
Volume
14
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

A new method for the determination of the cysteine content of synthetic peptides is described. For this purpose the classical amino acid analysis is not suitable, as cysteine may undergo oxidative dimerisation to cystine during the hydrolysis or evaporation. The intact peptide was reacted with N‐ethylmaleimide (NEM) yielding a stable S‐(N‐ethylsuccinimido)‐cysteine derivative, which could be separated by RP‐HPLC and characterised by mass spectrometry. For the quantitative determination less than 0.1 µM peptide was sufficient. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.

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