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A simple method for cloning leishmanial promastigotes

✍ Scribed by David A. Evans; Valerie Smith

Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
274 KB
Volume
72
Category
Article
ISSN
1432-1955

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For immunodiagnosis of kala-azar enzyme-linked immunosorbent assay (ELISA) and immunofluorescence testing (IFAT) are commonly used. In IFAT, whole parasite antigen and in ELISA the soluble antigen have been used. Preparation of ELISA antigen has certain inherent difficulties. We have developed a sim