A simple method for affinity purification and PCR amplification of poly(A)+ mRNA
β Scribed by Steven A. Sheardown
- Book ID
- 113289542
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 156 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0168-9525
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π SIMILAR VOLUMES
## SUMMPARY A simple, efficient method was used to purify QCR primers which had degraded during storage at -2O"C/-9O'C. The primers were electrophoresed ou 3 A (w/v) agarose gel, the main band was electroeluted via a trough cut in the gel. The primers were recovered by isobutanol extraction follo
In the present study a new DNA extraction method is described. The new protocol, which uses caprylic acid for isolating DNA, is technically simple and very fast, as it enables us to obtain DNA from peripheral blood in only 10 minutes. Moreover, DNA preparations obtained with this Kev words: DIS80 lo