A simple LC-MS/MS method to determine plasma and cerebrospinal fluid levels of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in patients with neurocysticercosis
✍ Scribed by Iliana González-Hernández; María Isabel Ruiz-Olmedo; Graciela Cárdenas; Helgi Jung-Cook
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 412 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.1659
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✦ Synopsis
ABSTRACT
The development and validation of an LC‐MS/MS method for the simultaneous determination of albendazole metabolites (albendazole sulfoxide and albendazole sulfone) in human plasma are described. Samples of 200 μL were extracted with ether–dichloromethane–chloroform (60:30:10, v/v/v). The chromatographic separation was performed using a C~18~ column with methanol–formic acid 20 mmol/L (70:30) as the mobile phase. The method was linear in a range of 20–5000 ng/mL for albendazole sulfoxide and 10–1500 ng/mL for albendazole sulfone. For both analytes the method was precise (RSD < 12%) and accurate (RE <7%) with high recovery (>90%). The method was successfully applied to determine the plasma and cerebrospinal fluid levels of albendazole sulfoxide and albendazole sulfone in patients with subarachnoidal neurocysticercosis who received albendazole at 30 mg/kg per day for 7 days. This LC‐MS/MS method yielded a quick, simple and reliable protocol for determining albendazole sulfoxide and albendazole sulfone concentrations in plasma and cerebrospinal fluid samples and is applicable to therapeutic monitoring. Copyright © 2011 John Wiley & Sons, Ltd.