𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple fluorometric assay for quantifying the adhesion of tumour cells to endothelial monolayers

✍ Scribed by Elizabeth A. Price; Deirdre R. Coombe; J. Clifford Murray

Book ID
104634515
Publisher
Springer
Year
1995
Tongue
English
Weight
861 KB
Volume
13
Category
Article
ISSN
0262-0898

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✦ Synopsis

A static adhesion assay employing 6-carboxy-3',6'-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of turnout cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF~. In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells.

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