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A simple fast micromethod for measuring enzyme activities which release tritium as tritium water

✍ Scribed by Walter L. Hughes


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
358 KB
Volume
161
Category
Article
ISSN
0003-2697

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✦ Synopsis


The entire procedure is carried out in a counting vial by mixing the reagents as a 20-to 30-1.11 drop in the cap of a counting vial, incubating, quenching the reaction, and then distilling the tritium water produced into the chilled vial, in which it is assayed after the addition of scintillation solvent and a clean cap. The application of this technique to the analysis of serum transaminases is described.