✦ LIBER ✦

A simple enzymatic method for the analysis of macromolecular fucose in biological materials

✍ Scribed by Thomas Maler

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 294 KB
Volume: 102
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90164-5

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✦ Synopsis

A sensitive enzymatic method employing L-fucose dehydrogenase has been used for the measurement of the amounts of fucose in 100-500 pg of plasma membrane protein and lo-100 pg of porcine submaxillary mucin. The assay showed linearity between 0 and 20 nmol of Lu-L-fucose when measuring NADH fluorescence. The fucose values obtained for the plasma membrane and submaxillary mucin correspond well with those obtained by gas-liquid chromatography.

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