An efficient method of separating proteins by electrophoresis with agarose gels containing sodium dodecyl sulfate is described. The separated proteins were then used to make antibodies. The separation of proteins in the molecular weight range 25K to 94K on 4 to 5% agarose gels showed good resolution
A simple electrophoretic procedure for separation of RNA on mixed agarose—acrylamide gel columns
✍ Scribed by Don P. Bourque; Aubrey W. Naylor
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 755 KB
- Volume
- 56
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A simple procedure is described for the preparation of mixed agarose-acrylamide gels for the separation of high molecular weight species of RNA by a modification of the method of PEACOCK AND DINGMAN~.
A method for the measurement of radioactivity in fractionated gels is described which permits tritium determination with efficiencies as high as 40 %.
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An exponential gradient gel with O-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins in a size-dependent fa