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A simple colorimetric method for detecting cell viability in cultures of eukaryotic microorganisms

✍ Scribed by Angelo Bonora; Donatella Mares

Publisher
Springer
Year
1982
Tongue
English
Weight
409 KB
Volume
7
Category
Article
ISSN
0343-8651

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We have devised a rapid method for examining the expression of a toxin gene following in vitro transfection using a bacterial beta-galactosidase (lacZ) gene as a reporter gene. Ricin A chain DNA and the lacZ gene, both under the control of the immunoglobulin gene promoter and enhancer, were transfec