A simple procedure for large-scale prepa
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M. Mukhopadhyay; N.C. Mandal
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Article
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1983
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Elsevier Science
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English
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A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu