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A simple binding assay for the direct determination of biotin in urine

✍ Scribed by Suman Mishra; Malina K. Storer; Catherine M.T. Sherwin; John G. Lewis

Book ID
116350226
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
111 KB
Volume
360
Category
Article
ISSN
0009-8981

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✍ Kalvin J. Gregory; Leonidas G. Bachas πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 92 KB

A competitive binding assay for biotin, biocytin, and desthiobiotin utilizing a genetically engineered enzyme-ligand conjugate is described herein. This assay is unique in that the enzyme-ligand conjugate consists of the streptavidin binding peptide Strep-tag II, which mimics the binding of biotin t