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A simple assay for the ribonuclease activity of ribonucleases in the presence of ethidium bromide

✍ Scribed by Tripathy, Debi Ranjan; Dinda, Amit Kumar; Dasgupta, Swagata

Book ID
120061389
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
551 KB
Volume
437
Category
Article
ISSN
0003-2697

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Ribonuclease A (RNase A) and the ribonuclease inhibitor protein (RI) form one of the tightest known protein-protein complexes. RNase A variants and homologues, such as G88R RNase A, that retain ribonucleolytic activity in the presence of RI are toxic to cancer cells. Herein, a new and facile assay i