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A simple and sensitive validated HPLC method for quantitative determination of cisplatin in human plasma

✍ Scribed by Kaushik, K. Harish; Sripuram, Vijay K.; Bedada, Satish; Reddy, Narsimha Y.; Priyadarshini, G. Indira; Devarakonda, Krishna R.


Book ID
126682015
Publisher
Informa plc
Year
2010
Tongue
English
Weight
768 KB
Volume
27
Category
Article
ISSN
1060-1333

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A simple and sensitive reversed-phase HPLC method with UV detection was developed and validated for the quantitation of 5-fluorouracil (5-FC) in human plasma. After acidification and salting out, 5-FU was extracted into ethyl acetate and back-extracted into a basic buffer. The extract was adjusted t