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A simple and rapid method of direct sequencing using Dynabeads

โœ Scribed by S. L. Thein; J. Hinton

Book ID
114712547
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
433 KB
Volume
79
Category
Article
ISSN
0007-1048

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A simple and rapid solid-phase RNA sequencing method has been developed based on Peattie's direct chemical method. 3'-Terminally labeled RNA was immobilized on DEAE-cellulose sheets and followed by specific modification with dimethyl sulfate, diethylpyrocarbonate, hydroxylamine (at pH 10 for the uri