A simple and rapid method for the purification of cytochrome P-450 (form LM4)
β Scribed by Michael A. Alterman; Andrew I. Dowgii
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 155 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0269-3879
No coin nor oath required. For personal study only.
β¦ Synopsis
A one step chromatographic method for the purification of membrane protein cytochrome P-450 LM4 has been developed and it indicates that CM Sepharose Fast-Flow is an excellent material for cytochrome P-450 LM4 chromatography.
π SIMILAR VOLUMES
We describe a straightforward and simple method for obtaining pure and active preparations of type 1 ribosome inactivating proteins (RIPs). The very high isoelectric point values, characteristic of these proteins, allow this purification in a single chromatographic step.
However, there were significant correlations between the enthalpy involved and each of VAG maximum viscosity, breakdown, viscosity at 50 Β°C and set back (r = 0.53, P < 0.05; r = 0.49, P < 0.05; r = 0.62, P < 0.05; r = 0.65, P < 0.01, respectively). For RVA data, correlations were found only between
## Abstract For Abstract see ChemInform Abstract in Full Text.