A General Method for Detecting and Sizin
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B.M. Barton; G.P. Harding; A.J. Zuccarelli
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Article
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1995
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Elsevier Science
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English
โ 639 KB
We have devised a method for detecting and estimating the sizes of large bacterial plasmids in the presence of genomic DNA by pulsed-field gel electrophoresis (PFGE). Bacteria harboring plasmids were embedded in agarose and lysed using a rapid protocol. Plugs were incubated with \(S 1\) nuclease and