✦ LIBER ✦

A simple and efficient hybridization technique for quantitating virus-specific ribonucleates in polyoma virus-infected cells

✍ Scribed by Perry d'Obrenan; Vera Chlumecky; John S. Colter

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 538 KB
Volume: 122
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90279-2

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✦ Synopsis

A rapid, convenient, and efficient hybridization method for the determination of virusspecific RNAs in Py virus-infected cells is described. The method involves carrying out the hybridization of viral RNAs present in the RNA isolated from infected cells directly on nitrocellulose filters carrying denatured, immobilized Py-DNA (15 ~1 RNA solution/25-mm2 filter). Under optimal conditions quantitative hybridization of viral RNA sequences is obtained within 24 h. The efficiency of hybridization is increased significantly when RNA fragmented by alkali under carefully controlled conditions is used.

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