A Simple and Direct Procedure for the Evaluation of Triglyceride Composition of Cocoa Butters by High Performance Liquid Chromatography - A Comparison with the Existing TLC-GC Method

✍ Scribed by Shukla, V. K. S. ;Schiøtz Nielsen, W. ;Batsberg, W.

• Publisher: John Wiley and Sons
• Year: 1983
• Weight: 434 KB
• Volume: 85
• Category: Article
• ISSN: 0931-5985
• DOI: 10.1002/lipi.19830850705

✦ Synopsis

A high performance liquid chromatography (HPLC) method forthe separation of triglycerides in cocoa butters at nanogram sensitivity is described. This procedureis based onUV detection at 220nm.Thisstudydernonstrates the feasibility of using UV detection system for the quantitation oftriglycerides employing a chromatographic separation system coupled with stop flow technique. HPLCanalysisoftriglycerides fromvariouscocoabutterscornpa- red well with values obtained by existing TLC-GC method. This appears to represent the first report to describe thequantitative analysisoftriglycerides employing short wavelength UV detection. The rapid quantitative analysis oftnglycerides by HPLC holds promiseinthequalitativeevaluationofcocoa butters