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A simple and cost-effective method for rapid genotyping of insertion/deletion polymorphisms

✍ Scribed by Renato Robledo; William Beggs; Patrick Bender

Book ID
117592329
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
139 KB
Volume
82
Category
Article
ISSN
0888-7543

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The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqu