A silica gel chromatographic procedure adapted to liquid-scintillation counting of 14c labelled organic acids from plant material and silage
✍ Scribed by J. R. Lessard; P. McDonald
- Publisher
- John Wiley and Sons
- Year
- 1966
- Tongue
- English
- Weight
- 695 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
A method is described for the separation of a wide range of organic acids present in plant material and in silage. The method consists of cold extraction with dilute sulphuric acid, followed by purification of the extract on a silica gel column. The organic acids in the purified extract were separated quantitatively using a continuous gradient-elution system of eluents through a small silica gel column.
The extraction method was found to be more efficient for extracting citric. oxalic and fumaric acids from red clover, than a standard method with ethanol, water and ion-exchange resins.
Most of the acids of the tricarboxylic acid cycle were detected in red clover. An unidentified acid accounted for more than 37 % of the organic acidity.
The activity of 14C-labelled acids could be measured directly on the eluate from the column in combination with a liquid scintillation system.