## Abstract Human parvovirus B19 infections are common in the general population, and infection during pregnancy may cause __hydrops fetalis__ and fetal death. To initiate adequate treatment, accurate laboratory diagnosis is essential. The most sensitive tests are nested PCR systems, but these assa
A serious parvovirus B19 genotype 2 infection not detected in a commercial PCR assay
✍ Scribed by Modi, Shookdev L.; Firnhaber, Cindy; Kana, Vibha; Skhosana, Lindiwe; Carmona, Sergio; Rossouw, Inéz
- Book ID
- 122222510
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 454 KB
- Volume
- 60
- Category
- Article
- ISSN
- 1386-6532
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## Abstract Parvovirus B19 (B19V) is divided into three genotypes. Genotypes 2 and 3 may cause diagnostic difficulties and their epidemiology is not well understood. In the present study the prevalence of B19V genotypes in patients with symptomatic infection in Poland was evaluated and the course o
Ninety-eight samples from 80 cases of spontaneous abortions after fetal death or hydrops fetalis from 12,000 pregnant women were examined using PCR. DNA was extracted from amniotic fluid, fetal blood, ascitic fluid and fetal biopsies or placenta specimens using QIA amp kits (QIAGEN). A 270-bp length