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A series of vectors to construct lacZ fusions for the study of gene expression in Schizosaccharomyces pombe

✍ Scribed by Marı́a J Lafuente; Thomas Petit; Carlos Gancedo

Book ID
117108560
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
476 KB
Volume
420
Category
Article
ISSN
0014-5793

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A gene bank of partial Sau3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248', which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. Th