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A sensitive sandwich enzyme immunoassay for γ-seminoprotein and its application to sex discrimination of blood and bloodstains

✍ Scribed by N. Yukawa; T. Kohno; E. Ishikawa; K. Takahama

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 919 KB
Volume: 55
Category: Article
ISSN: 0379-0738
DOI: 10.1016/0379-0738(92)90121-c

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✦ Synopsis

A sensitive sandwich enzyme immunoassay for gamma-seminoprotein (p30, prostate-specific antigen) is described for sex discrimination of blood and bloodstains. A polystyrene ball coated with rabbit anti-gamma-seminoprotein IgG was incubated with gamma-seminoprotein and, after washing, with affinity-purified rabbit anti-gamma-seminoprotein Fab'-horseradish peroxidase conjugate. Peroxidase activity bound to the polystyrene ball was assayed by fluorometry using 3-(4-hydroxyphenyl)propionic acid as hydrogen donor. The detection limit of gamma-seminoprotein was 0.15 pg per assay. Blood levels of gamma-seminoprotein, measured using 1-10 microliters of blood, were at least 3.3-fold higher in male adults than in female adults. The ratio of gamma-seminoprotein in terms of pg to hemoglobin in terms of mg was significantly higher in male adults than in female adults. Thus, the measurement of gamma-seminoprotein or both gamma-seminoprotein and hemoglobin was useful for the discrimination of blood and bloodstains of male and female adults, although with some limitations.

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