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A sensitive radioimmunoassay for isepentenyladenosine

โœ Scribed by Saleem A. Khan; M.Z. Humayun; T.M. Jacob

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
266 KB
Volume
83
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A sensitive radioimmunoassay for the nucleoside isopentenyladenosine (iA) by using iA-specific antibodies and a nitrocellulose membrane filtration technique is described. The reliability of the method is demonstrated by using specific tRNAs of known structures for the estimation of iA in their digests. This assay can be used to quantitate minute amounts of iA in the presence of a large excess of other nucleosides. t This is No. 4 in the series entitled ~'Immunological studies on nucleic acids and their components"; No. 3 is Ref. (3).

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