A sensitive measurement of oxygen uptake rate using a optochemical sensor

✍ Scribed by Chung-Ming Chi; Leah N. Frautschy; Frank B. Cerra; Wei-Shou Hu

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 457 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00157378

✦ Synopsis

The ~eas~e~~~t 3f the specific oxygen uptake rate (OUR) of slow growing organisms using a small sample size is often hampered by the consumption of oxygen by the electrode used. Using a optochemical sensor we measured the OUR of carrot embryos with appro~mately 1000 cell clusters and of hybridoma with a~pro~mately lo6 cells. An OUR as low as 0.02 ,umolfh can be accurately measured.