A sensitive, low-cost ultraviolet strip-scanner for paper chromatograms
✍ Scribed by Denis M. Abelson
- Book ID
- 104146330
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 201 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
In amino acids the sensitivity is smaller because the amino group is neutralized by the carboxyl group. In methionine, however, this is not so because of the sulfide group.
The acid character of the -SO,-and NO,-groups also markedly reduces their sensitivity towards iodine.
In many cases the paper is colored permanently, showing the reaction to be irreversible. We have found that many samples exposed to iodine vapor will give several spots if subsequently chromatographed.
Conclzcsions
High sensitivity towards iodine vapor is shown by basic or reducing compounds (electron donors or Lewis bases)? while acids or oxidizing compounds are the least sensitive.
In most cases the spots can be ascribed to complex formation between the sample and the iodine.
Fat&tad da Qhnica, Gral. FZoves 2124, Montevideo (Urqgzcay)
\R.