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A sensitive chemiluminescence method to measure the lipoxygenase catalyzed oxygenation of complex substrates

✍ Scribed by Dagmar Kratky; Achim Lass; Peter M. Abuja; Hermann Esterbauer; Hartmut Kühn

Book ID
117049814
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
241 KB
Volume
1437
Category
Article
ISSN
1388-1981

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## Abstract A fast and sensitive procedure to determine the turnover of the H2 hydrogen of lactate and quantify its ^2^H‐enrichment by ^1^H NMR is illustrated using C6 cells metabolizing (3‐^13^C) lactate in 50% ^2^H~2~O (vol/vol). ^2^H substitution of the lactate H2 hydrogen resulted in two easily