A sensitive atomic fluorescence system for metals in biological systems

✍ Scribed by S.A. Clyburn; G.F. Serio; B.R. Bartschmid; J.E. Evans; Claude Veillon

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 560 KB
• Volume: 63
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90209-2

✦ Synopsis

An atomic fluorescence spectrometric system for trace elemental determinations in biological samples is described. A heated graphite atomization furnace is used, with continuous sample introduction. Carbonic anhydrase and DNA dependent RNA polymerase enzymes are employed as application models, and accurate Zn determinations at the 10-r M level are made on enzyme samples of 0.1 mg and less with a precision of l-2%. The instrumentation is relatively simple. the system is versatile and has excellent stability.