✦ LIBER ✦

A sensitive assay for the reverse reaction of the first histidine biosynthetic enzyme

✍ Scribed by Jeanine Kleeman; Stanley M. Parsons

Book ID: 102628123
Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 251 KB
Volume: 68
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90700-9

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✦ Synopsis

A method capable of specifically detecting low levels of adenosine triphosphate phosphoribosyltransferase (EC 2.4.2.17) is given. It is based on conversion of the usual product of the enzyme, Nl-(5'-phospho-D-ribosyl)adenosine triphosphate to ATP, which is detected by luciferase luminescence utilizing a liquid scintillation spectrophotometer. The assay is linear in enzyme concentration down to a lower limit of at least 55 pg enzyme/0.2 ml.

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