𝔖 Bobbio Scriptorium
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A sensitive and specific enzyme immunoassay for cyclomaltoheptaose and some derivatives

✍ Scribed by Christophe Créminon; Florence Pilard; Jacques Grassi; Bruno Perly; Philippe Pradelles

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
577 KB
Volume
258
Category
Article
ISSN
0008-6215

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✦ Synopsis

Antibodies were raised against cyclornaltoheptaose (@zyclodextrin), providing a highly sensitive enzyme immunoassay for &zyclodextrin and some derivatives with a detection limit of 120 pg/mL. Investigations of cross-reactivities with a wide variety of linear and cyclic maltooligosaccharides demonstrate that the antibodies are highly specific for cyclomaltoheptaose and a number of derivatives. The epitope is probably located on the secondary hydroxyl groups of the rim side. This enzyme immunoassay is shown to be suitable to detect cyclomaltoheptaose in urine and in plasma.

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