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A sensitive and specific colorimetric method for the determination of α,β-diaminopropionic acid and the Lathyrus sativus neurotoxin

✍ Scribed by S.L.N. Rao

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
595 KB
Volume
86
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive color reaction ofo-phthalaldehyde (OPT) with a$-diaminopropionic acid (DAP) is described. The reaction is used to determine specifically the DAP content in tissues ofthe rat injected with this amino acid. The method indicated the presence in the liver and kidney of a alkali labile metabolite of DAP. A procedure is also described to determine the Lathyrus sativus neurotoxin @-N-oxalyl-L-Qdiaminopropionic acid (ODAP) in tissues ofthe rat injected with the neurotoxin and also its content in the seeds. The procedure is rapid and would be of use in studies with DAP and the L. sativus neurotoxin.

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