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A sensitive and simple method for the study of oxaloacetate compartmentation in isolated hepatocytes

โœ Scribed by E.A. Siess; D.G. Brocks; O.H. Wieland


Book ID
115906803
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
312 KB
Volume
70
Category
Article
ISSN
0014-5793

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A method for isolation of rat hepatocytes using liver perfusion by ethylenediamine tetra-acetic acid (EDTA)-containing sucrose solution and mechanical tissue disaggregation by controlled vibration (MVD) is described. The yields of hepatocytes produced by this method were similar to those obtained us