A sensitive and selective liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of actinomycin-D and vincristine in children with cancer

✍ Scribed by James I. Lee; Jeffrey M. Skolnik; Jeffrey S. Barrett; Peter C. Adamson

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 256 KB
• Volume: 42
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1211

✦ Synopsis

Abstract

We describe a selective and a highly sensitive assay for actinomycin‐D (Act‐D) and vincristine (VCR) in plasma employing high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) detection. The intraday precision (as defined by the coefficient of variation, CV) based on the standard deviation of replicates of quality control samples ranged from 4.9 to 7.5% and 6.5 to 11.3% with accuracy ranging from 90.7 to 98.1% and 91.2 to 103% for Act‐D and VCR, respectively. The interday precision ranged from 7.2 to 10.0% and 11.3 to 13.0% and the accuracy ranged from 94.3 to 102% and 90.7 to 91.6% for Act‐D and VCR, respectively. Stability studies showed that Act‐D and VCR were stable both during the assay procedure and long‐term storage. The lower limit of quantitation (LLOQ) for both Act‐D and VCR was 0.05 ng/ml. The analytical method showed excellent sensitivity, precision, and accuracy. This method is robust and is being successfully employed in a pharmacokinetic study of these agents in children with cancer, and is expected to support several ongoing and future pediatric trials. Copyright © 2007 John Wiley & Sons, Ltd.