A sensitive and rapid HPLC-ECD method for the simultaneous analysis of norepinephrine, dopamine, serotonin and their primary metabolites in brain tissue

✍ Scribed by Mario E. Alburges; Neelam Narang; James K. Wamsley

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 556 KB
• Volume: 7
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130070605

✦ Synopsis

This report details a very sensitive, rapid and accurate ion-pair HPLC-ECD method for the analysis of biogenic amines and their metabolites in brain tissue. The method described enables detection of picogram amounts of 3methoxy-4-hydroxy phenethyleneglycol (MHPG) (37 pg), 3,4-dihydroxy phenylacetic acid (DOPAC) (18 pg), norepinephrine (NE) (12 pg), epinephrine (E) (6 pgj, 5-hydroxyindoleacetic acid (5-HIAA) (18 pg), 3,4dihydroxyphenylethylamine (DA) (6 pgj, 3-methoxy-4-hydroxyphenylacetic acid (HVA) (12 pg) and 5hydroxytryptamine (5-HT) (12 pg). The linearity of the method is from 18.75 ng/mL to 300 ng/mL for MHPG; 9.37 to 150 ng/mL for DOPAC and 5-HIAA; 6.25 to 100 ng/mL for NE, HVA and 5-HT; and from 3.12 to 100 ng/ mL, for E and DA. The rcproducihilitg, expressed as coefficient of variance (CV%) within-run and between-run groups, was 2.25% and 19.49% for MHPG; 3.84"/0 and 29.84% for DOPAC; 0.89% and 8.97% for NE; 1.26% and 5.61% for E; 1.07% and 28.77%) for 5-HIAA; 2.65% and 10.65% for DA; 5.97% and 24.38% for HVA; and 4.44% and 9.45% for 5-H'T.