Crude cornea1 collagenase was obtained from ulcerating rabbit corneas by previously described methods (3). Semipurified cornea1 collagenase (fraction II, molecular weight 245,000
A semiquantitative enzyme assay for corneal collagenase
โ Scribed by J.U. Prause
- Book ID
- 114920473
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 110 KB
- Volume
- 53
- Category
- Article
- ISSN
- 1755-375X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A continuously recording spectrophotometric assay has been developed for Clostridium histolyticum collagenase based on the hydrolysis of 2-furanacryloyl-L-leucylglycyl-L-prolyl-L-alanine (FALGPA). The hydrolysis of this peptide by collagenase obeys Michaelis-Menten kinetics with V = 1.8 X IO5 pkatal
A simple protein determination method is described which gives a linear response from 0.05 to 3-4 mg/ml (or more) of protein and is suitable for dilute (<0.05 mglml) protein solutions if concentrated on paper by repeated spotting. Five-microliter solutions of a standard protein and the unknown are s