Determination of picomole quantitites of
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Kenneth G.D. Allen; Ching-Jang Huang; Catherine L. Morin
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Article
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1990
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Elsevier Science
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English
β 477 KB
A procedure to quantitate picomole amounts of hydroperoxides based on GSSG formation is described. Hydroperoxides are incubated with GSH and glutathione peroxidase, and the GSSG formed is measured by a GSSG-specific glutathione reductase recycling assay. Prior to analysis the remaining GSH is remove