N-linked oligosaccharides in glycoproteins are considerably more stable to mild alkali than are O-linked chains has been reexamined. A number of 'H-labeled model glycoproteins (fetuin, transferrin, and glycophorin) were treated with: (i) 0.05 M OH--l M BH; at 50Β°C for 16 h, (ii) 0.1 M OH--O.8 M BH,
A selective method for sequential splitting of O- and N-linked glycans from N,O-glycoproteins
β Scribed by Leonid M. Likhosherstov; Olga S. Novikova; Varvara A. Derevitskaya; Nikolay K. Kochetkov
- Book ID
- 107725965
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 753 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0008-6215
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