## Abstract ## Background Hematopoietic stemβcellβdirected gene transfer has achieved limited success in transducing clinically relevant levels of target cells. The expansion of geneβmodified cells is one way to circumvent the problem of inefficient transduction with current vectors. To this end,
A selective amplifier gene for tamoxifen-inducible expansion of hematopoietic cells
β Scribed by Ruifang Xu; Akihiro Kume; Kant M. Matsuda; Yasuji Ueda; Hiroshi Kodaira; Yoji Ogasawara; Masashi Urabe; Ikunoshin Kato; Mamoru Hasegawa; Keiya Ozawa
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 173 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1099-498X
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## Abstract ## Background __In vivo__ expansion of geneβmodified cells would be a promising approach in the field of hematopoietic stem cell gene therapy. To this end, we previously developed a selective amplifier gene (SAG), a chimeric gene encoding the granulocyte colonyβstimulating factor (GβCS
A clonal cell line, 1-1ras1000, transformed by the activated c-Ha-ras oncogene, does not form metastases after i.v. injection into mice (experimental metastasis assay). Here, we show that this cell line is useful as a recipient to detect metastasis-inducing genes, using a transfection assay. Cells (