A secreted mucin carrying Sialyl-Lewis a from colon carcinoma cells binds to E-selectin and inhibits HL-60 cell adhesion
✍ Scribed by Ke Zhang; Dan Baeckström; Gunnar C. Hansson
- Book ID
- 102869397
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 796 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Sialyl
-Lewis x and a are known as ligands for E-selectin (ELAM-I) involved in leukocyte-endothelial adhesion. L-Can& (light cancer antigen), secreted by a colon carcinoma cell line COLO 205, is a soluble mucin-type glycoprotein expressing sialyl-Lewis a antigens. L-Can4 was purified from spent culture medium by trichloracetic acid precipitation and Superose 6 gel filtration. With a monoclonal antibody against E-selectin (BBAI) as a positive control, the purified L-CanAg was shown to bind to E-selectin-Fc coated into plastic microtiter wells and to the surface of transiently E-selectin-transfected COS-I cells in a Ca2+-dependent way. lmmunofluorescent double labelling showed that both BBAl and L-Can& stained the same cells and morphological co-localization on E-selectin-transfected COS-I cells. Like BBAl, L-Can4 can inhibit leukocyte HL-60 cell adhesion to E-selectin-transfected COS-I cells, and this inhibition can be blocked by a F(ab'), fragment directed against the sialyl-Lewis a epitope.