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A Second DNA Methyltransferase Repair Enzyme in Escherichia coli

✍ Scribed by G. William Rebeck, Susan Coons, Patrick Carroll and Leona Samson


Book ID
123635749
Publisher
National Academy of Sciences
Year
1988
Tongue
English
Weight
918 KB
Volume
85
Category
Article
ISSN
0027-8424

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The polC (= dnaE) temperature-sensitive DNA polymerase III mutation from Escherichia coli BT1026 has been transduced into E. coli WP2 (to give CM731) and WP2 uvr A (to give CM741). In excision-deficient CM741 UV-induced Trp+ mutations progressively lost their photoreversibility during post-irradiati

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The photoreversibility of UV-induced mutations to Trp+ in strain Escherichia coli WP2 uvrA trp (unable to excise pyrimidine dimers) was lost at different rates during incubation in different media. In Casamino acids medium after a short initial lag, photoreversibility was lost over about one generat