✦ LIBER ✦

A screening technique to isolate bacterial strains producing high molecular weight bacteriocins

✍ Scribed by A. Jabrane; J. Destain; P. Compère; L. Ledoux; C-M. Calberg-Bacq; P. Thonart

Book ID: 104643939
Publisher: Springer-Verlag
Year: 1994
Tongue: English
Weight: 463 KB
Volume: 8
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00151482

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✦ Synopsis

Bacterial strains producing high molecular weight bacteriocins can be easily and rapidly screened in two-steps procedure. The first one uses the lysogenic property of bacteriocin production and the identification of the lysogenic cells by simple calorimetric detection of alkaline phosphatase in the culture medium after mitomycin C treatment. The presence of high molecular weight bacteriocins is determined in the second step by examination in transmission electron microscopy. This procedure is tested with 302 different strains, 3 of them are identified as high molecular weight bacteriocins producers.

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