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A Screening Technique for the Isolation of Bacterial Mutants Sensitive to Ionizing Radiation

โœ Scribed by J. Borsa and J. W. Hunt

Book ID
124597082
Publisher
Bioone
Year
1968
Tongue
English
Weight
873 KB
Volume
34
Category
Article
ISSN
0033-7587

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โœ Danchin, Antoine ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 541 KB

An E. coli strain deleted in the region malasd is used for the selection of conditional or auxotrophic mutants. Thermosensitive and auxotrophic strains have thus been isolated on plates. After selection in liquid medium, a strain has been isolated which is sensitive to excess one-carbon metabolites.